mouse anti panck monoclonal antibody  (Cell Signaling Technology Inc)

Journal: Journal of Nanobiotechnology

Article Title: Fucoidan-decorated metal-zoledronic acid nanocomplexes suppress tumor metastasis by inducing ferroptotic cell death and enhancing cancer immunotherapy

doi: 10.1186/s12951-025-03473-0

Figure Lengend Snippet: FTZ@Fu inhibits tumor metastasis and attenuates the immunosuppressive TME. ( A ) Hematoxylin and eosin (H&E) staining of spontaneous lung metastasis from orthotopically implanted 4T1-LM cells with different MNCs formulations treatments. Bar = 100 μm. Quantification of metastatic tumor colonies is shown. ( B ) 3D images showing tumor (PanCK) and vasculature (CD31) in mice lung. Enlarged pictures indicate micro- (blue area) and macrometastasis (pink area). ( C ) Representative images of the expressions of Ki67, γH2AX, IFN-γ, and granzyme b in nuclei (DAPI) in tumor tissues. Quantitative data are shown in the right panel. Bar = 100 μm. ( D ) Representative images of Treg and CD8 T-cell infiltration in tumor tissues by MNC treatment. Quantitative data are shown in the right panel. Bar = 100 μm. * p < 0.05; ** p < 0.01; and *** p < 0.001

Article Snippet: Each lung tissue sample was labeled with 40 μL of mouse anti-PanCK monoclonal antibody (Cell Signaling, #4545), followed by 40 μL of goat anti-CD31 polyclonal antibody (R&D Systems, AF3628).

Techniques: Staining

Journal: iScience

Article Title: Metabolic alterations and immune heterogeneity in gastric cancer metastasis

doi: 10.1016/j.isci.2025.112296

Figure Lengend Snippet:

Article Snippet: GC tissue sections were sequentially stained with various primary antibodies, including ATP5MC2 (Abcam, AB180149), PanCK (Cell Signaling Technology, 4523S), CD3 (Abcam, AB307134), CD20 (eBioscience, 2608794), SMA (Abcam, AB184675), PD1 (HUABIO, ET1606-41), CTLA4 (HUABIO, HA721269), LAG3 (HUABIO, HA721358), and TIM3 (HUABIO, EM1701-19).

Techniques: Virus, Knockdown, Plasmid Preparation, Over Expression, Recombinant, CCK-8 Assay, SYBR Green Assay, Purification, ATP Assay, Sequencing, RNA Extraction, Real-time Polymerase Chain Reaction, Software